Journal: Cardiovascular Diagnosis and Therapy
Article Title: Inhibition of ERK or Akt ameliorates intimal hyperplasia via up-regulation of Cx37 and down-regulation of Cx43 in balloon injury rat model
doi: 10.21037/cdt-20-345
Figure Lengend Snippet: Inhibition of ERK and Akt reduced neointima formation and cell proliferation in the balloon-injured rat model. (A) Representative H&E staining photomicrographs of carotid arteries for NI rats and untreated, MK2206-treated, and U0126-treated BI rats on day 14 surgery. Magnification 200×. (B) Quantification of the intima-to-media ratio. (C) Representative PCNA immunostaining 14 days post carotid ligation in NI, BI, MK2206-treated BI and U0126-treated BI rats. Magnification 200×. (D) Quantitative analysis of the luminal area. (E) Quantitative assessment of PCNA positive area over the total carotid area. *, P<0.05; **, P<0.01, versus BI rats. #, P<0.05; ##, P<0.01, versus NI rats. Bar graph indicates the mean ± SEM. BI, balloon injury; NI, not injured; PCNA, proliferating cell nuclear antigen.
Article Snippet: Microwave-heating was applied for antigen retrieval, and nonspecific blocking was performed by 4% normal goat serum plus 1% bovine serum albumin (BSA) in phosphate-buffered saline for 30 min. Then, an anti-rat PCNA rabbit polyclonal antibody (1:100; Santa Cruz Biotechnology Inc., Santa Cruz, CA) was used to incubate the sections.
Techniques: Inhibition, Staining, Immunostaining, Ligation